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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: H19 knockdown suppresses proliferation and induces apoptosis by regulating miR-148b/WNT/β-catenin in ox-LDL -stimulated vascular smooth muscle cells

Fig. 5

WNT1 was a target of miR-148b. a The predicted binding sequences between miR-148b and WNT1 3’UTR region, together with mutant sites in the WNT1-MUT reporter. b Luciferase activity was detected 48 h after transfection in HA-VSMCs co-transfected with WNT1-WT or WNT1-MUT reporter and miR-con, miR-148b, miR-con+pcDNA3.1 empty vector, or miR-148b + pcDNA-H19. c The effects of H19 knockdown (si-H19), miR-148b forced expression (miR-148b), and H19 overexpression (pcDNA-H19) on WNT1 protein expression were assessed using western blot assay at 48 h after transfection in HA-VSMCs. d WNT1 expression in serums of healthy volunteers (n = 40) and AS patients (n = 40). e and f The correlation analysis between WNT1 and H19 or miR-148b in 40 cases of AS patient serums. Data are presented as mean ± SEM (n = 3) of at least three independent experiments. *P < 0.05

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