Fig. 6

miR-148b modulated proliferation and apoptosis through WNT/β-catenin signaling in HA-VSMCs. (a and b) HA-VSMCs transfected with si-con+anti-miR-con, si-WNT1 + anti-miR-con, si-con+anti-miR-148b or si-WNT1 + anti-miR-148b were stimulated with 50 μg/ml ox-LDL for 24 h, followed by the evaluation of WNT1, β-catenin, C-myc and E-cadherin expressions. (C-H) HA-VSMCs transfected with anti-miR-con or anti-miR-148b were treated with or without XAV939 (10 μM) for 10 h and then stimulated with 50 μg/ml ox-LDL for 24 h, followed by the determination of cell proliferative ability (c), colony formation capability (d), PCNA and ki-67 expressions (e), apoptotic cell percentage (f), caspase-3 activity (g), as well as Bax and Bcl-2 expressions (h). Data are presented as mean ± SEM (n = 3) of at three independent experiments.*P < 0.05