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Table 5 Role of CRISPR/Cas9 in cancer modeling

From: CRISPR/Cas9: the Jedi against the dark empire of diseases

Type of cancer Method of CRISPR/Cas9 delivery Conclusion Reference
Pancreatic cancer Transfection based multiplexed delivery into mice Editing of multiple gene sets in pancreatic cells of mice [54]
Acute myeloid cancer (AML) Lentiviral based delivery into Hematopoirtic stem cells Loss of function in nine targeted genes analogous to AML [56]
Liver cancer Hydrodynamic injection into wild type mice Mutation in the Pten and p53 genes leading to liver cancer in mice [59]
Breast cancer Plasmid transfection into JygMC (mouse cell line) The stem cell marker Cripto-1 was shown to be as a breast target [60]
Pancreatic cancer Lentivirus/Adenovirus based delivery intosomatic pancreatic cells of mice Knockout of gene Lkb1 [61]
Lung cancer Plasmid transfection into human cell line (HEK 293) Chromosomal rearrangement among EML4 and ALK genes [62]
Lung cancer Lentivirus/Adenovirus mediated Gain of function of KRS and loss of function of p53 and Lkb1 [63]
Colon cancer Plasmid transfection into DLD1 and HCT116 cell lines (human) Loss of function in protein kinase c subgroups [64]
Colorectal cancer Electropolation into organoids intestinal epithelium (human) Loss of function and directed mutation in APC, SMAD4, TP 53 and KRAS genes [65]
Gliobastoma
Medulloblastoma
Postnatal PEI-mediated transfection and in utero electroporation into mice Deletion of TSGs (Ptch1, Trp 53, Pten and Nf1) [66]
Renal cancer Renca (mouse cell line) Knockout of TSG VHL to induce cancer [67]