Fig. 2

BAIBA ameliorates LPS-induced inflammation in differentiated 3 T3-L1 cells through AMPK-mediated pathway. a Western blot analysis of AMPK phosphorylation and adiponectin expression in differentiated 3 T3-L1 cells treated with BAIBA (0–30 μM) for 10 days. b Western blot analysis of LPS-induced NFκB and IκB phosphorylation in siRNA for AMPK transfected differentiated 3 T3-L1 cells treated with 10 μg/ml LPS and BAIBA (0–30 μM) for 10 days. Culture media analysis of (c) TNFα and (d) MCP-1 in siRNA for AMPK transfected differentiated 3 T3-L1 cells treated with 10 μg/ml LPS and BAIBA (0–30 μM) for 10 days. Means ± SEM were calculated data obtained from three independent experiments. ^***P < 0.001, ^**P < 0.01, and ^*P < 0.05 when compared to the control.^!!!P < 0.001,^!!P < 0.01, and^!P < 0.05 when compared to the LPS treatment. ^##P < 0.01, and ^#P < 0.05 when compared to the LPS plus BAIBA treatment