Fig. 2
MPT0B098 inhibits hypoxia-induced EMT in OEC-M1 cells. a The effect of MPT0B098 onhypoxia-induced HIF-1αexpression. OEC-M1 cells were treated with various concentrations, indicated as fold of IC50 values, of MPT0B098 for 18 h under hypoxic conditions. At the end of the drug treatment, cell lysates were prepared and analyzed by SDS-PAGE and Western blot. β-Actin was used as an internal control. b Each bar depicts the mean of the relative intensity of HIF-1α from three independent experiments. c The effect of MPT0B098 on hypoxia-induced EMT.Cells were treated with MPT0B098 at a concentration of 0.5-fold IC50 for 48 h under hypoxic conditions and then cell morphology was examined by crystal violet staining. Cells in normoxia were used as controls