Fig. 6

MPT0B098 downregulatesTGF-β/Smad signalingin OEC-M1 cells. a Cells were treated with various concentrations,indicated as fold of IC₅₀ values, of MPT0B098 for 36 h under hypoxic conditions. b Each bar depicts the mean of the relative intensities of phospho-Smads and Smads from three independent experiments (* p < 0.05, compared to hypoxia control). c Cells were treated with various concentrations,indicated as fold of IC₅₀ values, of MPT0B098 for 18 h, followed by an addition of 5 ng/mL TGF-β for another 18 h under hypoxic conditions. At the end of the drug treatment, cell lysates were prepared and analyzed by SDS-PAGE and Western blot. GAPDH was used as an internal control. d Each bar depicts the mean of the relative intensities of phospho-Smads and Smads from three independent experiments (* p < 0.05, compared to hypoxia control)