Fig. 1

SP110 regulates NF-κB-driven transcription. a HEK293T cells were co-transfected with pGL3-p5 × κB-F.Luc, pSV40-R.Luc, and the indicated constructs, and the relative firefly (F.)/Renilla (R.) luciferase (Luc) values for a basic promoter with 5 × κB sites were measured 2 days after co-transfection. The empty vector negative control is shown on the left. b HEK293T cells were co-transfected with pGL3-TNFα promoter-F.Luc, pSV40-R.Luc, and the indicated constructs, and the relative Luc values for the TNFα promoter were measured 2 days after co-transfection. The empty vector negative control is shown on the left. c and d HEK293T cells were co-transfected with pGL3-TNFα promoter-F.Luc, pSV40-R.Luc, and the indicated constructs, and the relative Luc values for the TNFα promoter were measured 2 days after co-transfection. The empty vector negative control is shown on the top. In (a and b), a, b, and c correspond to SP110a, SP110b, and SP110c, respectively. In (c and d), schematic representation of SP110 deletion mutants is shown on the left. The data are presented as the mean ± SD. Statistical significance of the difference between two sample groups was calculated using a two-tailed unpaired t-test. *P < 0.05; **P < 0.01; ***P < 0.001. The experiments were done at least twice