Fig. 3

Identification of nuclear localization signals in SP110. a Structure of the SP110 protein, showing the positions of the nuclear localization signals (NLSs), and schematic representation of eGFP fusion proteins that contain fragments of the SAND domain of SP110. b and c HEK293T cells were transfected with the indicated constructs, and the disparate localization of eGFP fusion proteins containing NLSs (b) and the SAND fragments (c, upper panels) was visualized by confocal microscopy at 2 days post-transfection. Scale bars: 10 μm. d H1299 cells were transfected with the construct expressing the eGFP-SP110b fusion protein, and the nuclear and nucleolar localizations of the protein were visualized by confocal microscopy at 2 days post-transfection. The upper and lower panels are representative of the eGFP-SP110b fusion protein that is localized in the nucleoplasm and the nucleolus, respectively. Scale bars: 10 μm. The cells were probed with an anti-nucleolin antibody to identify nucleoli (b and d) and subjected to Hoechst staining to identify nuclei (b, d, and lower panels in c). All data represent at least 2 independent experiments