Fig. 3

Avicequinone B sensitized anoikis in human lung cancer cells. a The reduction of viability was early observed in lung cancer H460 cells after culture under detachment condition for 6 h. b Avicequinone B at 4 μM significantly induced anoikis in H460 cells that were culture under detachment condition for 24 h. c Bright blue fluorescence of Hoechst33342 indicated DNA condense and apoptosis body was indicated in H460 cells treated with 4 μM of avicequinone B. d Detachment-induced apoptosis was evidenced with histograms obtained from flow cytometry analysis of H460 cells at anchorage culture (control attachment), detachment without treatment for 24 h (control) and detachment with 4 μM of avicequinone B for 24 h. e The detection early and late apoptosis without necrosis cells was remarkably increased after incubation of non-adherent H460 cells with avicequinone B at 4 μM. *p ≤ 0.05 versus untreated control cells at the same time point