Fig. 2
Illustrates the PFC (a & c) and HP (b & d) neurodevelopmental expression of NKCC1 (upper panel) and KCC2 (lower panel) mRNA between Control and Pb2+ exposed rats. Perinatal Pb2+ exposure resulted in an upregulation of NKCC1 in the PFC at PND 2, whereas in the HP NKCC1 was slightly upregulated at PND 2 and down regulated at PND 7. At PND 2 and 14 KCC2 was upregulated by Pb2+ exposure. However, Pb2+ exposure caused a down regulation of KCC2 mRNA expression at PND 2 and 7, followed by an upregulation at 14 in the HP. Data are presented as ± SEM and Tukey’s post hoc analyses are denoted as a significant difference in Control rats (p < 0.05*, p < 0.01**, p < 0.001***) as a function of Age, and denoted as a significant difference between Pb2+ vs. Control (p < 0.05#, p < 0.01##, p < 0.001###) as a function of Age and an Age X Treatment interaction for each developmental time-point (p < 0.05‡, p < 0.01‡‡, p < 0.001‡‡‡)