Skip to main content
Fig. 5 | Journal of Biomedical Science

Fig. 5

From: IL-33 receptor (ST2) deficiency downregulates myeloid precursors, inflammatory NK and dendritic cells in early phase of sepsis

Fig. 5

ST2 deficiency enhances early apoptosis of CD11c+ cells in sepsis. a The percentage of active caspase-3 positive spleen cells is assessed by immunohistochemistry. The results are presented as a mean percentage of positive stained nuclei per field (5 random fields, 4–5 tissues per group). Representative images show active caspase-3 positive nuclei in spleen of septic ST2−/− and WT mice (magnification at × 400). b The percentage of early apoptotic Annexin V+PI spleen cells is assessed 12 h after CLP by anti-mouse Annexin V antibody and PI staining. c Representative plots denote the percentage of early apoptotic Annexin V+PI cells of total spleen cells in septic ST2−/− and WT mice. d The percentage of early apoptotic Annexin V+PI spleen cells among CD11c+ cells. e Representative plots denote the percentage of early apoptotic Annexin V+PI cells of CD11c+ spleen cells in septic ST2−/− and WT mice. The percentage of early apoptotic Annexin V+PI spleen cells among B cells (CD19+) (f), macrophages (F4/80+) (g) and T cells (CD3+) (h) was shown. Data are presented as mean ± SE, n = 4–7 mice per group. *p < 0.05, **p < 0.01

Back to article page