Fig. 5

ST2 deficiency enhances early apoptosis of CD11c⁺ cells in sepsis. a The percentage of active caspase-3 positive spleen cells is assessed by immunohistochemistry. The results are presented as a mean percentage of positive stained nuclei per field (5 random fields, 4–5 tissues per group). Representative images show active caspase-3 positive nuclei in spleen of septic ST2^−/− and WT mice (magnification at × 400). b The percentage of early apoptotic Annexin V⁺PI⁻ spleen cells is assessed 12 h after CLP by anti-mouse Annexin V antibody and PI staining. c Representative plots denote the percentage of early apoptotic Annexin V⁺PI⁻ cells of total spleen cells in septic ST2^−/− and WT mice. d The percentage of early apoptotic Annexin V⁺PI⁻ spleen cells among CD11c⁺ cells. e Representative plots denote the percentage of early apoptotic Annexin V⁺PI⁻ cells of CD11c⁺ spleen cells in septic ST2^−/− and WT mice. The percentage of early apoptotic Annexin V⁺PI⁻ spleen cells among B cells (CD19⁺) (f), macrophages (F4/80⁺) (g) and T cells (CD3⁺) (h) was shown. Data are presented as mean ± SE, n = 4–7 mice per group. *p < 0.05, **p < 0.01