Fig. 2

Growth curves of double mutants. a Growth rate of mutants in media supplemented with ADS, all double mutant display a growth defect relative to their parent single mutants and the wild-type (WT) strain. b Growth rate of mutants in media supplemented with OADC, all double mutant display a growth defect relative to the wild-type (WT) strain c Growth curves of the ΔegtA-mshA double mutant relative to its parent strains in media supplemented with ADS. d Growth curves of the ΔegtA-mshA double mutant relative to its parent strains in media supplemented with OADC e Growth curves of the ΔegtB-mshA double mutant relative to its parent strains in media supplemented with ADS f Growth curves of the ΔegtB-mshA double mutant relative to its parent strains in media supplemented with OADC. g Growth curves of the ΔegtD-mshA double mutant relative to its parent strains in media supplemented with ADS. h Growth curves of the ΔegtD-mshA double mutant relative to its parent strains in media supplemented with OADC. i Growth rate of the complemented strain of ΔegtA-mshA relative to the wild-type in media supplemented with ADS. j Growth rate of the complemented strain of ΔegtA-mshA relative to the wild-type in media supplemented with OADC. Data represent 2–3 experiments. Statistical analyses were performed with Prism using a multiple t-test approach, assuming a uniform distribution with alpha set to 0.05 *(P < 0.05), **(P < 0.01), ***(P < 0.001)