Skip to main content
Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Incomplete cellular reprogramming of colorectal cancer cells elicits an epithelial/mesenchymal hybrid phenotype

Fig. 1

Reprogrammed CRC-iPC colonies show ESC-like features and pluripotency. a Morphology and expression of pluripotency markers of the CRC-iPC clones, iHCT-15 and iSK-CO-1. The presence of the pluripotency markers was determined by immunofluorescence staining. b Directed-differentiation of iPCs into the trilineage germ layers. For mesoderm lineages, Alizarin Red S and Oil Red O staining were performed; for ectoderm- and endoderm, immunofluorescence detection was used. c Spontaneous differentiation of CRC-iPCs. Floating embryoid bodies (EB) were attached and cultured under primary culture conditions to form post-iPCs. Images of the parental CRC morphology are also shown for comparison with that of the post-iPC cells. Scale bars, 100 μm (a-c) and 50 μm in oil red O staining of the CRC-iPC cell lines. d Expression of germ-layer markers in iPCs and the derived post-iPCs. Data of clone 5 (C5) and 2 (C2) of HCT-15 and SK-CO-1, respectively, are shown. The real-time RT-PCR values were calculated relative to the parental cancer cells and represented as mean ± SEM for triplicate experiments. *p < 0.05 and **p < 0.01

Back to article page