Fig. 2

Down-regulated but reversible expression of pluripotency genes on CRC reprogramming and re-differentiation. a RT-PCR detection of expression of the exogenous OSKM transgenes. Two independently isolated iPC clones, designated by the prefix “C”, were used. b RT-PCR analysis of endogenous expression of selected pluripotency genes in the parental cancer cell lines, and in H9 embryonic stem cells, which was used as a references. In both (a) and (b), RNA preparations of iPC cells at passage 25 or later were used. c Relative expression levels of the pluripotency genes. Expression was quantified by real-time RT-PCR, and the data presented were mean ± SEM from three independent experiments in triplicates. Expression levels of pluripotency genes were normalized to that of the respective parental CRC, which was arbitrarily set as 1.0, *p < 0.05 and **p < 0.01