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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells

Fig. 2

TNS2 interacts with Axl and up-regulates its downstream signaling and cellular glucose import. Effects of TNS2 and Axl expression on downstream signaling. a HEK293T cells were transfected with 2 μg Axl or 2 μg TNS2 or both (1 μg Axl with 1 μg TNS2) followed by Western blot analysis 24 h later. b, c Western blot analyses were performed in U2OS cells 24 h after transfection with TNS2 expression plasmid with or without shAxl plasmid. Recombinant human Gas6 were treated at 400 ng/mL for 15 min where indicated. Representative Western blot results are shown here and experiments were performed in triplicate. Quantitative data of the pTNS2/TNS2 ratios and the IRS-1 expression were presented in bar graphs. Data were obtained from three independent experiments and were presented as mean ± SEM. *p < 0.05. d After transfection with Axl or shAxl or vector control, U2OS cells were pre-incubated in growth medium without glucose for 1 h and then treated with 2-NDBG for 10 min followed by flow cytometry analysis

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