Fig. 4

Effect of metformin on mitochondrial function in HeLa cells. a HeLa cells were incubated for 20 h with 5 mM metformin, after which the cellular OCR was measured in XF24 bioenergetic assays. b HeLa cells were incubated for 16 h with the indicated concentrations of metformin, after which JC-1 staining was analyzed using flow cytometry. c Changes in FL1-H and FL2-H were further evaluated using a FACS Calibur flow cytometer. The results are representative of two independent experiments