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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology

Fig. 1

Autoubiquitination of Znf179 in the presence of E2 conjugating enzyme UbcH5 family proteins in vitro. a 293 T cells transfected with different doses (0, 1, 2 μg) of Flag-mZnf179 for 48 h were immunoprecipitated with anti-Znf179 antibody and analyzed by Western blotting with anti-Ubi and anti-Znf179 antibodies. b Total brain lysates from wild-type or Znf179-knockout mice were immunoprecipitated and analyzed by Western blotting with an anti-Znf179 antibody. c 293 T cells were transfected with Flag-mZnf179 for 48 h. The immunoprecipitated Flag-mZnf179 proteins were introduced to the mixture of purified E1, ubiquitin, Mg2+-ATP and one of several purified His-E2 enzymes to perform in vitro ubiquitination assays. The ubiquitination patterns of Znf179 were detected by Western blotting with anti-ubiquitin and anti-Znf179 antibodies

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