Fig. 1
From: L-caldesmon alters cell spreading and adhesion force in RANKL-induced osteoclasts
RANKL-induced differentiation of RAW264.7 cells. a Characteristic TRAP-stained RAW264.7 cells with RANKL induction for 5 days. Multinucleated OCs were observed by TRAP and nuclei staining with DAPI. b OCs characterized with actin ring formation around the cell periphery by using F-actin fluorescent staining with rhodamine phalloidin (red) and immuno-fluorescent staining β-actin (green). c Actin ring structure showing the core as indicated by # in RANKL-induced OC cells stained with l-CaD (right top) and phosphorylated l-CaD (p-l-CaD; right bottom), F-actin (middle), and merged color micrograph showing l-CaD staining (left top) and p-l-CaD (left bottom) in green, F-actin in red, and colocalized stains in yellow. Calibration bars in (a), (b), and (c) as indicated, respectively. d Actin ring structure composed of the core as indicated by # (labelled with F-actin as red in the top middle panel) and the peripheral rim as indicated by * (labelled with vinculin as green in the top left panel) and merged color micrograph (the top right panel) showing the actin ring as indicated by white arrow. Magnified portion (right bottom) showing the actin ring structure with the peripheral rim labelled with vinculin around the core in the center with red F-actin staining. Calibration bar: 20 μm as indicated in each panel