Fig. 3
From: L-caldesmon alters cell spreading and adhesion force in RANKL-induced osteoclasts
Effect of l-CaD overexpression on the colocalization of l-CaD/phosphorylated l-CaD to the F-actin associated with the actin ring structure in differentiated OCs. a Laser scanning confocal microscopy showing the images of RANKL-induced actin ring structures stained with l-CaD (green, top) and with F-actin (red, middle) in OCs transfected with EGFP and EGFP-l-CaD vectors, respectively. The actin core as indicated by * showing the F-actin rich staining with rhodamine phalloidin (red) at the periphery of the cells and actin ring as indicated by # showing the colocalized portion with the yellow core in the center. Calibration bar 50 μm as indicated. b The stacking confocal micrographs showing phosphorylated l-CaD staining in green (top), F-actin staining in red (middle), and merged images in orange or yellow (bottom). The actin ring and actin core as indicated showing the colocalized portion with the phosphorylated l-CaD and F-actin in the periphery. Calibration bar 50 μm as indicated