Fig. 5
From: L-caldesmon alters cell spreading and adhesion force in RANKL-induced osteoclasts
Effects of l-CaD on mechanical changes of cell spreading in RANKL-induced cells. a The height sensor image obtained with AFM scanning cell surface topography of height. Using the bearing analysis (built-in software of NanoScope Analysis v1.40), bearing area (Z) versus the bearing height (X) was adjusted to find the threshold for obtaining the full masking with blue on the whole cell (left panel). The threshold was used to define the minimized cell height at the cell boundary (X0, Z0), and set the maximal area bearing within the cell, and then normalize the relation curve describing the bearing area (%) to the bearing height (%) for the cell of interest. With increasing the height from 20 to 40% accompanied by increasing the bearing area as indicated from the cell periphery (middle panel) toward the center (right panel) of the cell. b Pseudo-coloring the cell of interest was used to find the peripheral area of the cell in the yellow coloring zone, with the percentage of bearing area (Zyellow) and cell height (Xyellow) as indicated. The bearing area covering the cell peripheral region (yellow zone) was used to estimate the tendency of cell spreading for the cells. c The 3D presentation for the height sensor image obtained with AFM scanning cell surface topography of height in RANKL-induced cells treated with si Ctr or si-l-CaD, or transfected with EGFP control or EGFP-l-CaD. d Quantitative analyses for the l-CaD dependent changes of the relation curve of normalizing bearing area (Zyellow) and cell height (Xyellow) (top), and the cell spreading (bottom) in cells treated with si Ctr or si-l-CaD, or transfected with EGFP control and EGFP-l-CaD. The values are the mean ± SEM (n = 6), with *, # indicating a significant difference compared to the si Ctr-treated or EGFP-transfected cells, respectively