Fig. 6

Effects of l-CaD on mechanical changes of adhesion force in RANKL-induced cells. (a) After AFM imaging the mechanical property of the cell surface, the recorded image at the adhesion mode was analyzed for the force curves at pixels of interest (i.e. the region outside the cell, at the cell periphery, and the region inside the cell), as indicated by *. The adhesion force at outside was used as the background adhesion force measurement. (b) The backward force vs tapping depth curves were used to calculate the adhesion force for cells transfected with EGFP or EGFP-l-CaD, or treated with si Ctr or si-l-CaD. (c) Quantitation of the l-CaD effect on the adhesion force at the region outside the cell, at the cell periphery, and the region inside the RANKL-induced cells with EGFP or EGFP-l-CaD(EGFP-WT), or with siCtr or si-l-CaD. The values are the mean ± SEM (n = 6), with *, # indicating a significant difference compared to the si Ctr-treated or EGFP-transfected cells, respectively