Fig. 6

In vitro function of AMC-HN-8 and TU177 cells with overexpression of ZNF667. a. MTS assays of cells transfected with pcDNA3.1 empty vector or pcDNA3.1-ZNF667, showing that cell transfected with pcDNA3.1-ZNF667 proliferate slower than that transfected with empty vector at the indicated time points. b. Clone formation assays of cells with or without overexpression of ZNF667. Cells overexpressing ZNF667 formed less clones than cells transfected with pcDNA3.1 empty vector. G. Transwell migration assays of AMC-HN-8 and TU177 cells were performed with or without ZNF667 overexpression. The migration ability of cells overexpressing ZNF667 decreased than cells transfected with pcDNA3.1 empty vector. H. Transwell invasion assays of AMC-HN-8 and TU177 cells were performed with or without ZNF667 overexpression. The invasion ability of cells overexpressing ZNF667 decreased than cells transfected with pcDNA3.1 empty vector. pcDNA3.1: the vector control group; ZNF667: ZNF667 over-expression group. The difference was statistically significant, **P < 0.01