Fig. 4

Deficiency in NFBD1 causes defective DSB repair and confers enhanced apoptosis to the PARP inhibitor olaparib (a) The repair of DNA damage was detected by comet assay. Tail moments were measured NPC cells treated with 4 μg/ml olaparib for 24 h, and the comet tail moment of 75 cells for each condition was quantified by CASP software and normalized to that of treatment with vehicle. Representative pictures are shown. NPC cells were treated with olaparib for 24 h, the apoptosis was determined by FCM (b) and hoechst 33342 staining (c). **P < 0.01 and ***P < 0.001