Fig. 5

The expression of NeuN from the CA1 Py layer is reduced following toxins treatments. NeuN immunofluorescence (a, c, e, g) and merge images between NeuN immunofluorescence and Hoechst histofluorescence (b, d, f, h, l). Control (a-b), LPS (c-d), Stx2 (e-f) and Stx2 + LPS (g-h) corresponding to the treatments of day 2. Merge images from a representative hippocampal control section between Gb3, GFAP immunofluorescences and Hoechst histofluorescence (i). Negative control by omitting primary antibody (j). NeuN expression in CA1 Py layer of all treatments at 2, 4, 7 and 20 days (k). Different letters (a, b, c, d) above the columns indicate significant differences between each column (k). The drawn rectangle was the area analyzed (l). The scale bar in Fig. J applies to all micrographs. Data were analyzed by One-way ANOVA, Bonferroni’s post hoc test, p < 0.05, n = 4