Fig. 6

Stx2 and Stx2 + LPS damage the myelin sheath. Micrographs of mouse brain hippocampus after 4 days of treatment with control, LPS, Stx2 or Stx2 + LPS (A-H). Immunofluorescence using an anti-MBP antibody (a, c, e, g). Merge images between anti-MBP immunofluorescence and Hoechst histofluorescence (b, d, f, h, k). Control-treated mice (a-b); LPS-treated mice (c-d); Stx2-treated mice (e-f) and Stx2 + LPS-treated mice (g-h). Negative control by omitting a primary antibody (i). MBP expression under all treatments at 2, 4, 7 and 20 days (j). Different letters (a, b, c, d) above the columns indicate a significant difference between each column (j). A low magnification micrograph shows different areas of the mouse brain: corpus callosum (cc); dorsal 3rd ventricle (D3V); cingulate cortex (cg); fields of the hippocampus (CA1, CA2, CA3 and DG); lateral ventricle (LV) and fimbria of the hippocampus (Fi); the drawn square in the Fi was the area analyzed (k). The scale bar in Fig. I applies to all micrographs. Data were analyzed by One-way ANOVA, Bonferroni’s post hoc test, p < 0.05, n = 4