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Fig. 7 | Journal of Biomedical Science

Fig. 7

From: Shiga toxin 2 from enterohemorrhagic Escherichia coli induces reactive glial cells and neurovascular disarrangements including edema and lipid peroxidation in the murine brain hippocampus

Fig. 7

Stx2 and Stx2 + LPS treatments increased the expression level of Iba1. Control (a-b), LPS (c-d), Stx2 (e-f) and Stx2 + LPS (g-h) after 2 days of treatments. Representative micrographs of anti-Iba1 immunofluorescence (a, c, e, g). Merge micrographs between anti-Iba1 immunofluorescence and Hoechst histofluorescence (b, d, f, h, k). Negative control by omitting a primary antibody (i). Iba1 expression levels at 2, 4, 7 and 20 days (j). The area analyzed was the drawn square in Rad area from the CA1 hippocampus (k). Different letters (a, b, c, d) above the columns indicate significant difference between different groups (j). The scale bar in fig. I applies to all micrographs. Data were analyzed by One-way ANOVA, Bonferroni’s post hoc test, p < 0.05, n = 4

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