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Table 1 Primers specific for rat inducible nitric oxide synthase (iNOS), and tumor necrosis alpha (TNF-α) and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), forward and reverse. F: Forward primer sequence. R: Reverse primer sequence

From: Effects of glucosamine against morphine-induced antinociceptive tolerance and dependence in mice

1

F(iNOS, Rattus)

GGGTCTTGTTAGCCTAGTCA

2

R(iNOS, Rattus)

TGTTGTTGGGCTGGGAATAG

3

F(TNFα, Rattus)

CCCAACAAGGAGGAGAAGTT

4

R(TNFα, Rattus)

GGCTTGTCACTCGAGTTTTG

5

F(GAPDH, Rattus)

AGCTCATTTCCTGGTATGACA

6

R (GAPDH, Rattus)

TTGCTCTCAGTATCCTTGCT