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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Promyelocytic leukemia zinc finger is involved in the formation of deep layer cortical neurons

Fig. 3

Reduced numbers of neurons in deep-layer cortex of Plzflu/lu mice. a, Dorsal view of whole-mount brains at P7. For the scale size, each of the thin lines indicates 1 mm, and the thick line indicates 5 mm. Quantifications of dorsal cortical surface area and cortical length shown in the lower panels. Statistical analysis performed using one-way ANOVA with appropriate post hoc tests: **p < 0.01 and ***p < 0.001. Plzfwt/wt, n = 7; Plzfwt/lu, n = 17; Plzflu/lu, n = 9. b, HE staining of sagittal brain sections, the higher magnification images of the black boxes were shown in the middle and right sides of the panel. Quantification of the area of cerebral cortex from rostral to caudal shown in the right panel. Statistics performed with Student’s t test: *p < 0.05; n = 3 per genotype. c, The left panel showed immunofluorescence images of Plzfwt/wt and Plzflu/lu mice brain sections. Tbr1 as layer VI neuron marker (green), Ctip2 as layer V neuron marker (red) and Hoechst33342 for nucleus staining (blue). For the middle panels, Satb2 as layer II-III marker (green). The higher magnification images of the yellow boxes were shown in the middle and right sides of panels. Quantification of the Tbr1+, Satb2+, and Ctip2+ neuron numbers in cerebral cortex shown in the right panel. Statistics performed with Student’s t test: *p < 0.05; n = 3 per genotype. Values represent the mean ± SEM. P, postnatal day; Plzfwt/wt, wild-type; Plzfwt/lu, heterozygous; Plzflu/lu, homozygous; Scale bar, 200 μm

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