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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Hepatitis B virus X protein (HBx) enhances centrosomal P4.1-associated protein (CPAP) expression to promote hepatocarcinogenesis

Fig. 3

CPAP expression increases HBx-induced cell growth and proliferation of HCC cells. a Proliferation of Hep3B and Hep3BX cells with ectopic expressed GFP or GFP-CPAP were determined by counting cell numbers every 24 h under monolayer culture conditions. b Cell proliferation was examined by BrdU incorporation assay in Hep3B cells with GFP-HBx/HA or GFP-HBx/HA-CPAP expression. The mean ± SD was obtained from three independent experiments (*, p < 0.05; **, p < 0.01). c Hep3B and Hep3BX cells with GFP or GFP-CPAP expression were subjected to colony-formation assay (upper) and in vitro trans-well migration assay (lower). d Hep3BX cells with CPAP siRNA (siCPAP) or control siRNA (siCtrl) were plated for colony-formation assay (upper) and in vitro trans-well migration assay (lower) as mentioned above (c). Hep3B with control siRNA (siCtrl) was used as a control. The number of cells was counted in five randomly selected fields. The data is presented as the mean ± SEM in three independent experiments (*, p < 0.05; **, p < 0.01; ***, p < 0.001)

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