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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Hepatitis B virus X protein (HBx) enhances centrosomal P4.1-associated protein (CPAP) expression to promote hepatocarcinogenesis

Fig. 4

CPAP augments TNF-α-mediated HBx stabilization in a NF-κB-dependent manner. a HCC cells with HA-CPAP or GFP-HBx were analyzed by Western blot analysis and RT-PCR. b Huh7 cells with HA-HBx and GFP or GFP-CPAP were treated with TNF-α (10 ng/ml) for 0, 1, and 2 h. The expression level of GFP, GFP-CPAP, and HA-HBx was determined by Western blot and/or RT-PCR as described in Fig. 1a. c Western blot analysis of Huh7 cells with GFP-CPAP/WT expression increased HA-HBx stability upon TNF-α treatment (10 ng/ml, 1 h) compared with GFP-CPAP/MT or GFP control. d Huh7 cells were transfected with non-targeting siRNA (Ctrl) or NF-κB/p65 siRNA (p65) for 24 h. The protein levels were examined by Western blot analysis after further transfection of HA-HBx and GFP or GFP-CPAP for an additional 24 h with TNF-α treatment for 1 h. e Huh7 cells were transiently transfected with HA-HBx and pSuper/CPAP or pSuper/NS control for 24 h. Protein expression of CPAP and HA-HBx was determined after treating cells with TNF-α for 1 h. The expression levels of HA-HBx are indicated as ratio, and three independent experiments were performed

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