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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Hepatitis B virus X protein (HBx) enhances centrosomal P4.1-associated protein (CPAP) expression to promote hepatocarcinogenesis

Fig. 5

Augmented NF-κB activity in CPAP-overexpressed HBx positive HCC. a-b HCC tissues with (a) or without (b) overexpressed CPAP and HBx were collected for Western blot analysis to detect the expression level (p65) and the activation status (p65/S536p) of NF-κB. GAPDH was used as a loading control. The expression levels of CPAP and HBx mRNAs in HCC were detected by Q-PCR, normalized by actin mRNA (NT, non-tumor part; T, tumor part). Total of 24 HCC tissues with (N = 15) or without (N = 9) overexpressed HBx/CPAP were analyzed, and two representative cases for both (a) and (b) are shown. c The correlation between CPAP/HBx expression and activated NF-κB was found to be statistically significant by Chi-square analysis; p = 0.015. d The expression level of CREB mRNA is determined in HBx-positive HCC by Q-PCR. A total of 33 specimens were enrolled, and the correlation between overexpressed CREB (T/NT > 1) and CPAP expression was analyzed by Mann Whitney U test (two-tailed); p = 0.020. e ROC (receiver operating characteristic curve) analysis of overexpressed CREB and CPAP in HBx-positive HCC from (D) (p = 0.021)

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