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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells

Fig. 4

TREM-1 promotes M1 macrophage polarization in line with increased M1 cytokine profile but not M2 in DSS-treated colonic macrophages. Lamina propria macrophages were isolated from colons of water- or DSS-treated WT and TREM-1 KO mice (n = 6/group) on day 7 post-DSS initiation. a Contour plot of FACS analysis data showed the gating strategy employed to identify distinct iNOS+ M1 macrophages among CD45+CD11b+MHCII+CD103CD11cF4/80+ cells. b Percentage and absolute number of iNOS+ macrophages as determined by intracellular staining in (a). Data are the mean ± SEM of two experiments. *, p < 0.05 or **, p < 0.01 by Mann-Whitney nonparametric test. c Real-time PCR analysis of mRNA levels of the indicated genes in the AutoMACS-purified F4/80+ colonic macrophages from the DSS-treated mice. Data are the mean ± SEM of two experiments and are expressed as fold change relative to GAPDH mRNA. *, p < 0.05 by Mann-Whitney nonparametric test

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