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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells

Fig. 5

Treatment with exogenous IL-22 or TREM-1-expressing macrophages rescues TREM-1-deficient mice from DSS-induced colitis. WT and TREM-1 KO mice were intraperitoneally injected with 500 ng IL-22 in PBS, or PBS alone, on every other day starting 1 day before (day − 1) initiating the standard DSS protocol. a Weight change, b survival rate, c colon length, d representative HE and AB-PAS staining, and e histopathological score were determined in DSS-treated WT mice, PBS + DSS-treated TREM-1 KO mice, and IL-22 + DSS-treated TREM-1 KO mice (n = 8/group). Data in a, c and e are the mean ± SEM and representative of two independent experiments. Statistical significance was determined by Mann-Whitney nonparametric test. *, p < 0.05; **, p < 0.01 or ***, p < 0.001 as indicated. Survival rate was compared using the log-rank test, *, p < 0.05. f-g Weight changes in (f) WT mice (n = 6/group) and (g) TREM-1 KO mice (n = 6/group) that were treated with DSS and intraperitoneally injected with by AutoMACS-purified F4/80+ peritoneal elicited macrophages (PECs) isolated from either WT or TREM-1 KO mice, as indicated. Data are mean ± SEM and representative of two independent experiments. h Representative images of histopathology of sections of colonic tissues that were prepared from DSS-treated WT and TREM-1 KO mice with intraperitoneally injected either WT or TREM-1 F4/80+PECs and stained with HE or AB-PAS. Scale bar: 100 μm. i Histopathological score were determined in DSS-treated WT mice with WT or TREM-1 KO F4/80+PECs adoptive transferred, and DSS-treated TREM-1 KO mice with WT or TREM-1 KO PECs adoptive transferred, respectively (n = 6/group)

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