Table 1 Sequences of primers used in this study
Gene | 5′ to 3′ end | Sequence |
|---|---|---|
GAPDH | F | GCATCCACTGGTGCTGCC |
R | TCATCATACTTGGCAGGTTTC | |
TREM-1 | F | GTCTCAGAAGTCAAAGCTGC |
R | GTCTGGTAGTCTCTGCCAAG | |
TNFα | F | CCTCACACTCAGATCATCTTC |
R | CGGCTGGCACCACTAGTTG | |
IL-6 | F | GCCTTCCCTACTTCACAAGT |
R | GAATTGCCATTGCACAACTCT | |
IFNγ | F | CTTCCTCATGGCTGTTTCTG |
R | TGTCACCATCCTTTTGCCAG | |
IL-1β | F | TTGAAGAAGAGCCCATCCTC |
R | CAGCTCATATGGGTCCGAC | |
IL-17a | F | GCTTCATCTGTGTCTCTGATG |
R | GCGCCAAGGGAGTTAAAGAC | |
IL-22 | F | TCCGAGGAGTCAGTGCTAA |
R | AGAACGTCTTCCAGGGTGAA | |
IL-23a | F | GCCTGGAACGCACATGCAC |
R | CCTTTGCAAGCAGAACTGGC | |
GMCSF | F | GAACCTCCTGGATGACATGC |
R | CAGTCCGTTTCCGGAGTTG | |
iNOS | F | CATTCTACTACTACCAGATCG |
R | GCAAAGAACACCACTTTACC | |
IL-12a | F | ACATGGTGAAGACGGCCAG |
R | GAAGTCTCTCTAGTAGCCAG | |
Arg-1 | F | GGGTGGAGACCACAGTCTG |
R | AGTGTTGATGTCAGTGTGAGC | |
YM-1 | F | TTATCCTGAGTGACCCTTCTAAG |
R | TCATTACCCTGATAGGCATAGG | |
IL-10 | F | ATGCAGGACTTTAAGGGTTAC |
R | CCTGAGGGTCTTCAGCTTC |