Fig. 1

Activation of TrkB by 7,8-DHF treatment improved long-term functional outcomes and reduced brain edema after ICH. a The experimental design scheme used to study the effect of TrkB activation by 7,8-dihydroxyflavone in mouse ICH. H: hemoglobin assay. B: behavioral tests. BWC: brain water content. WB: western blots. ELISA: enzyme-linked immunosorbent assay. CV: cresyl violet. FJB: Fluoro-Jade B. TUNEL: Terminal deoxynucleotidyl transferase dUTP nick end labeling. IHC: immunohistochemistry. Behavioral assessment included b mNSS, c rotarod latency, d beam latency, and e beam score. Values are mean ± S.E.M; ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs. vehicle group. (n = 12 mice / group, two-way ANOVA). f Brain edema was assessed by brain water content at 3 days post-ICH. Cont-BG: contralateral basal ganglia; Cont-CX: contralateral cortex; Ipsi-BG: ipsilateral basal ganglia; Ipsi-CX: ipsilateral cortex. Values are mean ± S.E.M; ^**P < 0.01 and ^***P < 0.001 vs. contralateral hemisphere; ^##P < 0.01 vs. vehicle group. (n = 7 mice / group, Student’s t-test) (g) Body weights were recorded up to 28 days post-ICH. Values are mean ± S.E.M; (n = 12 mice / group). h Hemorrhage size was assessed by hemoglobin assay at 3 days post-ICH. Values are mean ± S.E.M. (n = 6 mice / group, Student’s t-test) A.U.: arbitrary units; DHF20: 20 mg/kg 7,8-dihydroxyflavone; DHF40: 40 mg/kg 7,8-dihydroxyflavone