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Fig. 1 | Journal of Biomedical Science

Fig. 1

From: 5′-hydroxy Auraptene stimulates osteoblast differentiation of bone marrow-derived mesenchymal stem cells via a BMP-dependent mechanism

Fig. 1

5′-HA promotes the differentiation of mBMSCs into osteogenic cell lineage. Cytotoxicity of 5′-HA on cultured primary mBMSCs as measured by MMT assay (a) and Cell Titer-Blue® cell viability assay (b) after 3 days in culture. c Effect of 5′-HA (1–50 μM) on cell proliferation of cultured mBMSCs as measured by counting the cell number at different concentrations of 5′-HA after 5 and 10 days. d Dose-dependent stimulatory effect of 5′-HA on osteoblast differentiation of mBMSCs as measured by quantification of ALP activity after 7 days of induction and (e) quantitative Alizarin red staining for matrix mineralization after 12 days of induction. Cells were either cultured without osteogenic induction media (Ctrl, control), or induced to osteogenic lineage in the absence (0) or the presence of different concentrations of 5′-HA. Values were normalized to cell viability and represented as fold change over control non-induced cells. Stained images were shown for both ALP activity and Alizarin red staining. Values are mean ± SD of three independent experiments, (*p < 0.05, **p < 0.005 compared to control without 5′-HA for A&B; and compared to induced cells without 5′-HA for c&d)

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