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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: 5′-hydroxy Auraptene stimulates osteoblast differentiation of bone marrow-derived mesenchymal stem cells via a BMP-dependent mechanism

Fig. 2

5′-HA stimulates the mRNA expression of osteogenic markers in mBMSCs. a QPCR analysis of osteogenic markers expression in mBMSCs that induced to osteoblast differentiation without (control) or with 50 μM 5′-HA for 7 days. Each target gene was normalized to reference genes as described in M&M and genes were represented as fold changes over induced cells without 5′-HA. b Upregulation of different osteogenic-related genes in mBMSCs by 5′-HA (50 μM) as measured by osteogenic RT2 profiler array as described in M&M. Cells were induced to osteogenic lineage in absence or presence of 5′-HA for 7 days. Data were represented as percentage of categorized genes according to Table 1. Values are mean ± SD of three independent experiments, (*p < 0.05, **p < 0.005)

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