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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: 5′-hydroxy Auraptene stimulates osteoblast differentiation of bone marrow-derived mesenchymal stem cells via a BMP-dependent mechanism

Fig. 3

5′-HA does not affect the adipocyte differentiation of mBMSCs. a 5′-HA does not affect adipocyte differentiation of mBMSCs as measured by Oil red O staining and its quantification. Cells were either cultured without adipogenic induction media (Ctrl, control), or induced to adipogenic lineage in the absence (0) or the presence of different concentrations of 5′-HA. Images of Oil red O staining for fat droplets accumulation in differentiated mBMSCs were shown. b QPCR analysis of mRNA expression of adipogenic markers at day 12 of the differentiated mBMSCs into adipocyte with or without 5′-HA (50 μM). Each target gene was normalized to reference genes and represented as fold change over induced control (0). Values are mean ± SD of three independent experiments, (*p < 0.05, **p < 0.005)

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