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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: 5′-hydroxy Auraptene stimulates osteoblast differentiation of bone marrow-derived mesenchymal stem cells via a BMP-dependent mechanism

Fig. 5

5′-HA stimulates the differentiation of mBMSCs into osteobalst via activating BMP signaling pathway. a Western blot analysis of stimulating the phosphorylation of Smad1/5/8 in mBMSCs by 5′-HA (50 μM) versus control. b Western blot analysis of inhibiting BMP signaling pathway by specific BMP1R inhibitor, (LDN-193189, 10 μM) in absence and presence of 5′-HA. c Inhibitory effect of BMP1R inhibitor (LDN-193189, 10 μM) on the stimulatory effect of 5′-HA (50 μM) on BMP2-induced osteogenesis in mBMSCs as measured by quantitative ALP activity. d Dose-dependent stimulatory effect of 5′-HA on Smad4 mRNA expression and (e) SMAD4 protein expression in BMP2-treated mBMSCs as measured by qPCR and Western blot analysis. Cells were induced with BMP2 in the absence (0) or the presence of 5′-HA (different concentrations) for 24 h. Each target gene was normalized to reference genes and represented as fold change over induced control (0) without 5′-HA. Values are mean ± SD of three independent experiments, (**p < 0.005)

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