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Table 1 Up-regulation of osteogenic genes expression in mBMSCs treated with 50 μM of 5′-HA for 6 days during osteoblast differentiation

From: 5′-hydroxy Auraptene stimulates osteoblast differentiation of bone marrow-derived mesenchymal stem cells via a BMP-dependent mechanism

Gene name Gene Symbol Fold change
Ossification and matrix molecules
 Alkaline phosphatase, liver/bone/kidney Alpl 8.3
 Bone gamma carboxyglutamate protein Bglap 6.2
 Biglycan Bgn 4.5
 Collagen type I alpha 1 Col1a1 3.2
 Collagen type I alpha 2 Col1a2 2.3
 Collagen type V alpha 1 Col5a1 2.1
 FMS-like tyrosine kinase 1 Flt1  
 Secreted phosphoprotein 1 (Osteopontin) Spp1 5.3
BMP signaling pathway
 Bone morphogenetic protein 2 Bmp2 7.5
 Bone morphohenitic protein 4 Bmp4 4.2
 Bone morphogenetic protein 7 Bmp7 2.7
 Bone morphogenetic protein receptor. Type 1A Bmpr1a 2.1
 Bone morphohenitic protein receptor, type 1B Bmpr1a 3.4
 MAD homolog 5 (Drosophila) Smad5 2.8
Cell Adhesion Molecules
 Fibronectin 1 Fn1 4.3
 Integrin beta 1 (fibronectin receptor beta) Itgb1 3.2
 Integrin alpha 2 Itga2 4.2
 Integrin alpha 2b Itga2b 2.9
Osteogenic growth factors
 Fibroblast growth factor receptor 2 Fgfr2 2.8
 Insulin-like growth factor 1 Igf1 8.4
 Insulin-like growth factor I receptor Igf1r 4.3
 Platelet derived growth factor. Alpha Pdgfa 3.8
 Vascular endothelial growth factor A Vegfa 4.2
Osteogenic transcription factors
 Distal-less homeobox 5 Dlx5 6.4
 Runt related transcription factor 2 Runx2 5.9
 Sp7 transcription factor 7 Sp7 4.6
 Twist gene homolog 1 Twist1 4.3
  1. Cells were induced to differentiate into osteoblast without (control) or with 50 μM 5′-HA for 6 days. Mouse osteogenesis RT2 Profiler™ PCR array with 84 osteoblast genes was performed for each cDNA sample using the SYBR® Green quantitative PCR method. Up-regulated genes by BMSCs in the presence of 5′-HA were represented as fold change over control differentiated cells without 5’-HA