Table 1 Up-regulation of osteogenic genes expression in mBMSCs treated with 50 μM of 5′-HA for 6 days during osteoblast differentiation
Gene name | Gene Symbol | Fold change |
|---|---|---|
Ossification and matrix molecules | ||
Alkaline phosphatase, liver/bone/kidney | Alpl | 8.3 |
Bone gamma carboxyglutamate protein | Bglap | 6.2 |
Biglycan | Bgn | 4.5 |
Collagen type I alpha 1 | Col1a1 | 3.2 |
Collagen type I alpha 2 | Col1a2 | 2.3 |
Collagen type V alpha 1 | Col5a1 | 2.1 |
FMS-like tyrosine kinase 1 | Flt1 | |
Secreted phosphoprotein 1 (Osteopontin) | Spp1 | 5.3 |
BMP signaling pathway | ||
Bone morphogenetic protein 2 | Bmp2 | 7.5 |
Bone morphohenitic protein 4 | Bmp4 | 4.2 |
Bone morphogenetic protein 7 | Bmp7 | 2.7 |
Bone morphogenetic protein receptor. Type 1A | Bmpr1a | 2.1 |
Bone morphohenitic protein receptor, type 1B | Bmpr1a | 3.4 |
MAD homolog 5 (Drosophila) | Smad5 | 2.8 |
Cell Adhesion Molecules | ||
Fibronectin 1 | Fn1 | 4.3 |
Integrin beta 1 (fibronectin receptor beta) | Itgb1 | 3.2 |
Integrin alpha 2 | Itga2 | 4.2 |
Integrin alpha 2b | Itga2b | 2.9 |
Osteogenic growth factors | ||
Fibroblast growth factor receptor 2 | Fgfr2 | 2.8 |
Insulin-like growth factor 1 | Igf1 | 8.4 |
Insulin-like growth factor I receptor | Igf1r | 4.3 |
Platelet derived growth factor. Alpha | Pdgfa | 3.8 |
Vascular endothelial growth factor A | Vegfa | 4.2 |
Osteogenic transcription factors | ||
Distal-less homeobox 5 | Dlx5 | 6.4 |
Runt related transcription factor 2 | Runx2 | 5.9 |
Sp7 transcription factor 7 | Sp7 | 4.6 |
Twist gene homolog 1 | Twist1 | 4.3 |