Fig. 2

The expressions of RAGE and molecules involved in adhesion, migration and inflammation were significantly increased under hyperglycemic condition and were diminished in response to cilostazol treatment in a dose-dependent manner. a, densitometric quantification of Western blot bands (n = 3 /group). b, protein expressions of RAGE, MMP-2, VCAM-1, ICAM-1 and FAK were examined by Western blot analysis. A7r5 cells were incubated for 24 h with 100 and 200 μM cilostazol after stimulation with 5 and 30 mM glucose. Mannitol was used as an osmolality control. Western blots were independently repeated three times, and the representative data are shown. GAPDH served as a loading control. *, statistically significant differences, p < 0.05 by the Student’s t test. Error bars, SEM of three independent experiments