Fig. 3

The effects of hsa-miR-1975 mimic and sponge on WSN replication. a A549 cells were transfected with control SiRNA (Si_CTR) or hsa-miR-1975 mimic (mimic) and then infected with WSN (MOI = 0.1). At 24 h after infection, cells were harvested for immunoblotting of viral NP and cellular GAPDH proteins. The band intensities were quantified, and the relative NP/GAPDH ratios are shown below the blots. b A549 cells were transfected with control SiRNA (Si_CTR) or hsa-miR-1975 mimic (miR-1975 mimic) and then infected with WSN (MOI = 0.1). At 24 h p.i., cells were harvested and processed for RT-qPCR analysis. The NP expression levels were normalized by GAPDH. c and d, A549 cells were either transfected with control stuffer sponge or miR-1975 sponge and then infected with WSN (MOI = 0.1) for 24 h. Western Blots of viral NP and cellular GAPDH were performed. The numbers below Western Blot are NP protein ratio normalized by GAPDH (c). Relative NP vRNA levels were assessed by RT-qPCR and normalized by GAPDH (d). Values represent the mean ± SD of three independent experiments (b and d). Statistical comparisons between groups by Student’s t test (b and d). **P < 0.01, ***P < 0.001