Fig. 4

Expression of IFNB after hsa-miR-1975 mimic transfection. a A549 cells were not treated, pretreated with 1 μM MRT67307 for 30 min or pretreated with 4 μM ruxolitinib for 24 h. The cells were then transfected with control SiRNA (Si_CTR) or hsa-miR-1975 mimic as indicated. At 48 h post-transfection, A549 cells were infected with WSN (MOI = 1) for 6 h and then harvested. Cellular RNAs were extracted and measured by RT-qPCR. The levels of NP vRNA and IFNB mRNA were normalized by GAPDH mRNA. Values represent the mean ± SD of three independent experiments. b Expression of ISG15 and PKR mRNA were normalized by GAPDH mRNA from A549 cells that were treated as mentioned in (a). Values represent the mean ± SD of three independent experiments. c Vero cells were transfected with control SiRNA (Si_CTR) or hsa-miR-1975 mimic. At 48 h post-transfection, Vero cells were infected with WSN at a MOI of 1 for 6 h and then harvested. Cellular RNAs were extracted and measured by RT-qPCR. The levels of NP vRNA were normalized by GAPDH mRNA. Values represent the mean ± SD of three independent experiments. d Vero cells were infected with WSN at a MOI of 1 for 6 h and then harvested. Cellular RNAs were extracted and measured by RT-qPCR. Levels of endogenous miR-1975 and Y5 expression were normlized by U24. Values represent the mean ± SD of three independent experiments.Statistical comparisons between groups by one-way ANOVA with Bonferroni correction for multiple comparisons (a and b) and Student’s t test (c and d). *P < 0.05, **P < 0.01, and ***P < 0.001