Fig. 5

hsa-miR-1975 is packaged into exosomes. a Characterization of exosomes by Western Blot analysis. The A549 cell lysates and exosomes were immunoblotted with the indicated antibodies. b Exosomes pellets were diluted in 500 μL PBS. Size of exosomes were determined by using the dynamic light scattering technique (Zetasizer Nano ZS, Malvern Instruments, UK). c Relative expression of hsa-miR-1975 in exosomes after treating with 10 μM GW4869 or DMSO for 24 h. Values represent the mean ± SD of three independent experiments. d Relative expression of U24 in exosomes after treating with 10 μM GW4869 or DMSO for 24 h. Values represent the mean ± SD of three independent experiments. e Assessment of relative expression of hsa-miR-1975 in exosomes without WSN infection,with WSN infection (MOI = 1) for 24 h and with WSN infection (MOI = 1) and 10 μM GW4869 treatment for 24 h by RT-qPCR. Values represent the mean ± SD of three independent experiments. Statistical comparisons between groups by Student’s t test (c-d) and one-way ANOVA with Bonferroni correction for multiple comparisons (e). *P < 0.05, **P < 0.01 and ***P < 0.001