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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Exosome-delivered and Y RNA-derived small RNA suppresses influenza virus replication

Fig. 6

Exosome-delivered hsa-miR-1975 suppresses influenza virus replication. a Illustration of the experimental design to study the functional role of exosomally transferred hsa-miR-1975. Donor cells were transfected with control siRNA (Si_CTR) or hsa-miR-1975 inhibitor and then treated with mock or Poly(I:C). b Expression of hsa-miR-1975 in exosomes from donor cells after treateing with RNases was assessed by RT-qPCR. Values represent the mean ± SD of three independent experiments. c Expression of hsa-miR-1975 in recipient cells after receiving exosomes from donor cells as illustrated in (a) and then infected with WSN (MOI = 1) for 6 h. U24 was used for normalization of expression. Values represent the mean ± SD of three independent experiments. d Expression of IFNB mRNA in recipient cells after receiving exosomes from donor cells that were treated as mentioned in (c). GAPDH was used for normalization of expression. e The cellular RNAs were harvested and assessed by RT-qPCR for quantification of NP vRNA. GAPDH was used for normalization of expression. Expression of NP vRNA in recipient cells after receiving exosomes from donor cells that were treated as mentioned in (c). Values represent the mean ± SD of three independent experiments. Statistical comparisons between groups by one-way ANOVA with Bonferroni correction for multiple comparisons (b-e). *P < 0.05, **P < 0.01, and ***P < 0.001

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