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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Correction to: Reactive oxygen species-dependent mitochondrial dynamics and autophagy confer protective effects in retinal pigment epithelial cells against sodium iodate-induced cell death

Fig. 3

ROS mediate autophagy and exert a survival action in NaIO3-treated RPE cells. a After NaIO3 treatment as indicated cell lysates were used to determine LC3 and p62 by immunoblotting. b, c After treatment with Baf A1 (100 nM) and/or NaIO3 (30 mM) for 6 h, confocal microscopy was used to determine LC3II and Tom20. Scale bars indicated 10 μm. * p < 0.05, indicating significant effect of Baf A1, either in the absence or presence of NaIO3. d, e After treatment with drugs as indicated (i.e. 100 nM Baf A1, 3 mM 3-MA, 10 mM NAC, 30 μM Nec-1, 10 or 30 mM NaIO3) for 24 h, cell viability was determined. Data were mean ± S.E.M. from three independent experiments. * p < 0.05, indicating significant cytotoxic effect of NaIO3. # p < 0.05, indicating significant effects of Baf A1, 3-MA, and NAC on the action of NaIO3. f After treatment with NAC (10 mM) and/or NaIO3 (30 mM), LC3 protein was determined by immunoblotting

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