Fig. 1

Cytotoxicity of penfluridol in human acute myeloid leukemia (AML) cells harboring different Fms-like tyrosine kinase 3 (FLT3) statuses. a The chemical structure of penfluridol. b and c Three AML cell lines including HL-60 and U937 cells (wild-type FLT3), and MV4–11 (mutant FLT3, internal tandem duplication), were treated with vehicle or indicated concentrations of penfluridol for 24 and 48 h, respectively. Cell viability was measured using an MTS assay. Values are presented as the percentage of cell inhibition, where vehicle-treated cells were regarded as 100%. Data are presented as the mean ± SD of three independent experiments. The 50% growth inhibitory concentration (IC₅₀) values after 24- and 48-h treatment with penfluridol in each cell line are shown