Fig. 5

Exogenous MFG-E8 increases NF-κB activation in injured aged arteries. a–l CCAs of young and aged mice were ligated, with or without rMFG-E8 (2 μg/mL) treatment for 4 days. Representative dual immunofluorescence photographs of MFG-E8 (a, e, and i) and phosphorylated NF-κB p65 (Ser536) (b, f, j) in ligated CCA sections. The merged images depict an association (yellow) of MFG-E8 (red) and phosphorylated NF-κB p65 (Ser536) (green) (d, h, and l); bar, 50 μm. m CCAs of aged mice were ligated, with or without rMFG-E8 treatment for 4 days. Proteins were extracted from 10 pooled CCAs per group for Western blotting and examined using antibodies against NF-κB p65 phosphorylated at Ser536 and NF-κB p65