Fig. 7

MFG-E8 increases transcription of NF-κB-dependent proinflammatory genes in aged VSMCs. a–c VSMCs isolated from the aortas of aged mice were cultured with or without rMFG-E8 (250 ng/mL) for 24 h. a The protein expression of ICAM-1 and VCAM-1 in aged VSMCs was evaluated through immunoblotting. The quantitative analysis results for ICAM-1 (b) and VCAM-1(c) are normalized to Gapdh (n = 3). d–g VSMCs derived from young and aged mice were treated with 1% fetal bovine serum in either the presence or absence of rMFG-E8 for 16 h after starvation. The transcript expression of downstream genes of NF-κB including ICAM-1 (d), VCAM-1 (e), TNF-α (f), and iNOS (g) in VSMCs treated with rMFG-E8 was evaluated through quantitative real-time PCR (n = 3). Data are presented as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, one-way analysis of variance followed by Tukey’s multiple comparison test