Fig. 8

Exogenous MFG-E8 into carotid arteries dose not significantly increase ligation-induced vascular remodeling in aged mice. a–c Representative images displaying the cross-sectional areas of sham-operated (a) and ligated (b) carotid arteries of aged mice 21 days after ligation or sham operation. rMFG-E8 (2 μg/mL) was delivered using Pluronic gel into the CCAs of aged mice immediately after complete ligation of the vessels (c). Verhoeff–van Gieson staining was performed on all sections; bar, 100 μm. d–h Morphometric analyses of the areas of intima+media (d), intima (e), media (f), and lumen (h) and the I/M ratio (g) 21 days after ligation (n = 5–8 for each experimental group). Data are presented as mean ± SEM. *P < 0.05; one-way was analysis of variance followed by Tukey’s multiple comparison test